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類器官原代培養全流程
實驗前準備
CO2 培養箱、雙人單(dan)面超凈臺、倒置顯微(wei)鏡、臺式冷凍離心機、水(shui)浴鍋、水(shui)浴搖床,醫(yi)用冰(bing)箱、-80°冰(bing)箱、移(yi)液器(一(yi)套)、眼科(ke)剪、眼科(ke)鑷。
試(shi)劑:小(xiao)(xiao)腸(chang)類器(qi)(qi)官培(pei)養基,小(xiao)(xiao)腸(chang)類器(qi)(qi)官消化液(ye),小(xiao)(xiao)腸(chang)組(zu)織保存液(ye),小(xiao)(xiao)腸(chang)類器(qi)(qi)官緩(huan)沖液(ye),硫酸慶大霉素,雙抗,組(zu)織固定液(ye),基質膠,60mm細胞(bao)培(pei)養皿, 100μm濾篩,15ml離心管,1.5ml EP管若干,4度冰盒,眼科(ke)剪刀,眼科(ke)鑷。
實驗流程
(1)樣本準備:
用組(zu)織消(xiao)(xiao)化(hua)液在37℃進(jin)行消(xiao)(xiao)化(hua)10-15min(消(xiao)(xiao)化(hua)過程中隨時觀(guan)察消(xiao)(xiao)化(hua)情況)。
使(shi)用100μm孔徑的篩網進行過濾,取(qu)少量濾液在(zai)鏡下進行觀察,可以看到明顯(xian)的隱窩狀組織結構。
(4)離心、加膠
(5)鋪板
(6)觀察
歐瑪生物類器官培養基系列,華雅均有現貨,歡迎咨詢
產品(pin) 貨號 名稱(英文(wen))
結直(zhi)腸癌類器官(guan)培養(yang)基 OM11 (Human) Colorectal Cancer Organoid Culture Medium 100ml
人結(jie)腸類器官培養基 OM11N (Human) Colorectal Organoid Culture Medium 100ml
胃癌類器官培養基 OM12 (Human) Gastric Cancer Organoid Culture Medium 100ml
人(ren)胃類器(qi)官培(pei)養基 OM12N (Human) Gastric Organoid Culture Medium 100ml
肺癌類器官培(pei)養基 OM13 (Human) Lung Cancer Organoid Culture Medium 100ml
人肺類器(qi)官(guan)培養基 OM13N (Human) Lung Organoid Culture Medium 100ml
人胰腺癌類器官(guan)培養基 OM14 (Human) Pancreatic Cancer Organoid Culture Medium 100ml
人胰腺類器官(guan)培養基 OM14N (Human) Pancreatic Organoid Culture Medium 100ml
肝癌(ai)類器官培養(yang)基 OM15 (Human) Liver Cancer Organoid Culture Medium 100ml
人(ren)肝臟類器官培養(yang)基 OM15N (Human) Liver Organoid Culture Medium 100ml
宮頸癌類器官培(pei)養(yang)基 OM16 (Human) Cervical Cancer Organoid Culture Medium 100ml
人宮頸(jing)類器官培養基 OM16N (Human) Cervical Organoid Culture Medium 100ml
食管(guan)鱗癌類(lei)器官培養(yang)基 OM17 (Human) Esophageal Squamous Cancer Organoid Culture Medium 100ml
乳腺癌類器官培養基(ji) OM18 (Human) Breast Cancer Organoid Culture Medium 100ml
膀(bang)胱癌(ai)類器(qi)官培養基(ji) OM19 (Human) Bladder Cancer Organoid Culture Medium 100ml
小(xiao)鼠小(xiao)腸類器(qi)官培(pei)養基 OM31 (Mouse) Small Intestinal Organoid Culture Medium 100ml
小鼠大腸類器官培養基 OM32 (Mouse) Large Intestinal Organoid Culture Medium 100ml
小鼠肝臟(zang)類(lei)器官(guan)培(pei)養(yang)基(ji) OM33 (Mouse) Liver Organoid Culture Medium 100ml
小鼠胃類(lei)器官培養(yang)基(ji) OM34 (Mouse) Gastric Organoid Culture Medium 100ml
小鼠肺類器官培養(yang)基 OM35 (Mouse) Lung Organoid Culture Medium 100ml
小鼠子宮(gong)類(lei)器官培養基 OM36 (Mouse) Uterine Organoid Culture Medium 100ml
小(xiao)鼠胰腺類器官培養基 OM37 (Mouse) Pancreatic Organoid Culture Medium 100ml
小鼠食管類器官培養基 OM38 (Mouse) Esophageal Organoid Culture Medium 100ml
歐瑪生物類器官培養基系列,華雅均有現貨,歡迎咨詢